Sumlai Lozada Chang -Protein purification – Best Researcher Award

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Sumlai Lozada Chang -Protein purification – Best Researcher Award

Mrs. Sumlai Lozada Chang distinguished academic and researcher in the field Protein purification. 

🌐 Professional Profile

Educations📚📚📚

She completed her undergraduate studies with a Bachelor of Biochemistry (Licenciatura en Bioquímica) from the Faculty of Biology at the Universidad de La Habana in 1993. Later, she pursued postgraduate studies and earned a Master in Science and Technology of Biotechnological Processes (Maestría en Ciencias y Tecnología de Procesos Biotecnológicos) from the Institute of Pharmacy and Food at the Universidad de La Habana in 2015.

Course

She has pursued numerous courses to enhance her expertise. In 1994, she took a course on Biological Safety in Laboratories at Instituto Finlay, followed by a Basic Immunology Course in 1995, and Biometry and Experimental Design at the Faculty of Biology, UH, the same year. She studied Biochemistry of Fermentations in 1996 and attended courses on Protein Structures and Enzyme Immobilization. Between 1996 and 1998, she completed English Level I at Instituto Finlay, continuing to Levels II, III, and IV. In 1999, she attended an Industrial Chromatography Course by A. Pharmacia Biotech in Germany. She also studied Theory and Management Techniques at Instituto Finlay. In 2000, she took an Advanced English Course and a Basic Course on Microcomputer Operation at CENSAI, and studied Protein Purification and Isolation at the Faculty of Biology, UH, along with Ultra-cleanliness and Maintenance of Cleanroom Conditions at Instituto Finlay. She also learned about the Operation Cost System of Plant III, General Aspects of Tangential Filtration, and Biosafety at Instituto Finlay in 2001.

In 2002, she took a course on Good Manufacturing Practices for Biological Products at Instituto Finlay. She studied Philosophy at ISP “E. José Varona” in 2003 and English at CNIC the same year. She learned about the Milli-UF-50 Ultrafiltration System for Purified Water at Instituto Finlay in October 2003 and Technological Modifications for IFA Vaccine Production in 2004. She completed courses on Good Practices for Supervisors, Water in the Pharmaceutical Industry, and Specialist Categorization in Advanced Technology in 2005. In 2006, she took courses on Integral Security and Biosafety, Good Practices for Biological Products, and Good Practices in Documentation at Instituto Finlay. She studied Risk Management at Terra Farma in 2008 and Treatment of Non-Conformities with CAPA Focus at Instituto Finlay in 2008 and 2009.

In 2009, she also took courses on Training in Garment Change in Controlled Environments, Basic Validation Principles for Active Substance Area, Fermentation and Sanitization Operations, and Cleaning and Sanitization of Areas at Instituto Finlay. In 2010, she studied Integrated Management of Biosafety and Quality in Biopharmaceutical Products at Instituto Finlay. She took courses on the Implementation of Monitoring Programs in 2012, Handling and Operation of the AKTAprocess Chromatography System, Personnel Behavior in Classified Areas, Control Charts, and Applying Quality Control Charts at Instituto Finlay. In 2014, she attended a Technical Conference on Filtration and studied Process Validation at Instituto Finlay. She took a Protein Chromatography Course at CIM in April 2015, followed by an Initial Course on Good Manufacturing Practices at CIM in September 2016. Most recently, she attended the International Course of Bioprocess in 2023.

Professional Experience at Instituto Finlay

She has held various key positions throughout her career. From 1994 to 1996, she served as the Shift Head of the Purification Area, overseeing the purification of outer membrane vesicles (VME) of Neisseria meningitidis serogroup B. In 1996, she worked as an analyst in the Process Control Department, where she determined the content of proteins, polysaccharides, and lipopolysaccharides, analyzed molecular size by chromatography, and assessed protein expression through SDS-PAGE. From 1996 to 1997, she was the Lead Specialist for the extraction stage of VME. Between 1997 and 1999, she specialized in the purification of capsular polysaccharides, particularly the purification of N. meningitidis polysaccharide C and Salmonella typhi polysaccharide Vi.

In 2000 and 2001, she worked as a Process Analyst. From 2002 to 2004, she was the Head of the Support Group Area, responsible for the preparation of solutions and culture media, including material preparation and sterilization. She became the Head of the Purification Area from 2006 to 2007, where she led the purification of VME of N. meningitidis serogroup B, as well as the purification of N. meningitidis polysaccharides C and A, and Salmonella typhi polysaccharide Vi. She later directed the Department of Active Pharmaceutical Ingredients Production, focusing on the production of Neisseria meningitidis and Salmonella cultures

📝🔬Publications📝🔬

Marco A. Saraiva – Biophysics – Best Researcher Award

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Marco A. Saraiva – Biophysics – Best Researcher Award

Dr. Marco A. Saraiva distinguished academic and researcher in the field Biophysics. 

🌐 Professional Profile

Educations📚📚📚

He holds a Degree in Chemistry from the Faculty of Sciences of the University of Lisbon, which he completed in 2000 with a mark of 16/20. In 2008, he earned a PhD in Analytical Chemistry from the same institution

Work Experience

He has a diverse and extensive professional experience in the field of chemistry. In 1999/2000, he undertook his final year research project in reaction kinetics and mechanistics at the Faculty of Sciences of the University of Lisbon, under the supervision of Professor Lídia Miguel Pinho Chumbinho de Albuquerque and Professor Maria Luísa Calisto de Jesus Moita, earning a mark of 19/20. During this time, he gained experience in conductimetry and multiparametric analysis. In 2001/2002, he conducted a research project on the reactivity of DNA codified amino acids analogues at the School of Sciences, University of Minho, Portugal, supervised by Professor Hernâni Maia, and gained expertise in reverse phase high performance liquid chromatography (RP-HPLC). From 2003 to 2008, he pursued a PhD in Analytical Chemistry, focusing on mass spectrometry applied to the study of the Maillard reaction at the Department of Chemistry and Biochemistry, Faculty of Sciences of the University of Lisbon, under the supervision of Professor Maria Helena Florêncio and co-supervision of Professor Carlos Borges. He developed skills in electrospray ionization mass spectrometry (ESI-MS) and hyphenated techniques such as LC-MS and GC-MS. In 2008/2009, he conducted post-doctoral studies in Molecular Biophysics at the Department of Chemistry, Cambridge University, UK, under the supervision of Dr. Sophie E. Jackson, where he worked with ESI-MS (Q-TOF), absorption spectroscopy, fluorescence spectroscopy, circular dichroism, isothermal titration calorimetry, capillary electrophoresis, cell culture, and protein purification systems. From 2011 to 2016, he engaged in post-doctoral studies in photochemistry at the Center for Structural Chemistry, Instituto Superior Técnico, University of Lisbon, focusing on the structural and dynamic characterization of early stages in alpha-synuclein fibrillation in the presence of mannosylglycerate, under the supervision of Professor António L. Maçanita

Awards and Honours

He has received numerous grants and honors throughout his career. In 2000, he was awarded the Curricular grant PRODEP III/Measure 3/Action 3.2 from the Faculty of Sciences of the University of Lisbon, Portugal, for a duration of three months. He then received a PhD grant from the Portuguese Foundation for Science and Technology (FCT), Portugal, under the reference SFRH/BD/3162/2000. Following his PhD, he was granted a post-doctoral fellowship by the FCT under the reference SFRH/BPD/37016/2007. Later, he received another post-doctoral grant from the Mass Spectrometry National Network (RNEM), Portugal, with the reference RNEM/DGH/022125 in 2018. His professional recognition includes an invitation to become a member of the Royal Society of Medicine (United Kingdom) in 2022. In 2023, he was selected for the “International Young Scientist Awards” under the category of “Best Researcher Award” and was also nominated for Full Membership in Sigma Xi (USA).

Research Interests

His research interests are focused on the study of reaction processes in solution, particularly through the identification, characterization, and monitoring of species formed in physiologically relevant reaction mixtures. He primarily investigates the reaction of specific amine targets with α-dicarbonyls, a key aspect of the Maillard reaction. Additionally, he is interested in probing the fundamentals of electrospray ionization (ESI) mechanisms, exploring the fundamental aspects of ESI mass spectrometric techniques. He is also gaining interests in molecular biophysics, particularly in the study of molecular chaperone systems and α-synuclein, utilizing advanced spectroscopic and spectrometric techniques.

📝🔬Publications📝🔬

Fakher Frikha – Bioinformatics Structural – Top Researcher Award

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 Prof Dr. Fakher Frikha  distinguished academic and researcher in the Bioinformatic stuctural. Fakher’s research fields span Engineering, Computer Science, Biology, Biostatistics, Bioinformatics, and Biochemistry. He has expertise in Biochemistry, Proteins, Lipids, Enzyme Activity, Enzyme Production, Lipase, Laccase, Immobilization, Bioinformatics, Homology Modeling, Molecular Docking, QSAR, Drug Design, Molecular Dynamics Simulation, and Biostatistics. Currently, he works at the Department of Biology, University of Sfax as an assistant professor in bioinformatics and Biostatistics. His current project is mainly in the field of structural bioinformatics and biostatistics, including 3D structures molecular modeling of proteins, molecular dynamics, molecular Docking, study of the structure-function relationship, and design of experiments.

🌐 Professional Profiles

Educations📚📚📚

Fakher Frikha, a Ph.D. holder in Biological Engineering from the National School of Engineers of Sfax (ENIS), has been an Assistant Professor since 2012. His Ph.D. research focused on the molecular modeling of lipolytic enzymes. He also holds a Master’s degree and a National Engineering Degree from ENIS, and a Higher Technician degree in Public Health from the Higher School of Health Sciences and Techniques of Sfax (ESSTSS)

Research projects

Research projects:

  • Dissertation at ESSTSS Microbiology Service, Habib BOURGUIBA University Hospital, Sfax. Topic: “Peripheral samples from newborns: bacteriological analysis and interpretation.”
  • Final project at ENIS Enzymatic Lipolysis Unit, ENIS, Sfax. Topic: “Comparative study of bird lipases.”
  • Master’s thesis at ENIS Laboratory of Biochemistry and Enzymatic Engineering of Lipases, ENIS, Sfax. Topic: “Modeling 3-D structures of lipases.”
  • Doctoral thesis at ENIS Laboratory of Biochemistry and Enzymatic Engineering of Lipases, ENIS, Sfax. Topic: “Molecular modeling of lipolytic enzymes: study of structure-function relationships.”
  • Member of the Laboratory of Biochemistry and Enzymatic Engineering, ENIS, Sfax (2011).
  • Member of the Laboratory of Microbial, Enzymatic, and Biomolecule Biotechnology (LBMEB), Center of Biotechnology of Sfax (CBS), University of Sfax (2015).
  • Member of the Laboratory of Biochemistry and Enzymatic Engineering, ENIS, Sfax (2018).
  • Member of the Laboratory of Molecular and Cellular Screening Processes (LPCMC), Center of Biotechnology of Sfax (CBS), University of Sfax (August 2021).

Il semble que vous ayez une expérience variée et solide dans le domaine des sciences biologiques et de la recherche, ainsi que dans l’enseignement universitaire. Voici une reformulation de votre expérience professionnelle :

  • Juillet/Août 1999 : Technicien au Laboratoire d’analyse médicale au complexe IBN ANNAFIS, Sfax.
  • 2003 et 2004 : Réussi aux épreuves écrites de recrutement de technologue.
  • Du 15/09/2005 au 15/06/2006, Du 15/09/2006 au 14/09/2007, Du 15/09/2007 au 14/09/2008 : Contrats étudiants chercheurs à l’Institut Supérieur de Biotechnologie de Sfax.
  • À partir du 07/09/2009 : Assistant à la Faculté des Sciences de Sfax, spécialisé en Bioinformatique et Biostatistique (BMC3, SVE3, IAA3, Master BMC) – enseignement de cours théoriques et pratiques. En outre, vous avez assuré des vacations à l’ENIS-GB3 et GB2 en Biotechnologie & Agroalimentaire, ainsi que des travaux pratiques en bioinformatique et en méthodologie de la recherche expérimentale.
  • À partir du 07/09/2012 : Maitre-Assistant à la Faculté des Sciences de Sfax, toujours dans le domaine de la Bioinformatique et Biostatistique (BMC3, SVE3, IAA3, Master Recherche et Master Professionnel) – enseignement de cours théoriques et pratiques.
  • À partir de 2015 : Devenu responsable de la start-up Algreen, dont la mission est de convertir vers un usage parasismique. Cependant, le texte est ambigu, et il n’est pas clair ce que signifie “convert into a para use he”. Si vous pouvez fournir plus de détails sur les activités de la start-up, cela aiderait à clarifier cette partie.

Votre expérience montre une progression professionnelle cohérente dans votre domaine d’expertise, allant de la recherche et de l’enseignement universitaire à des responsabilités entrepreneuriales.

Professional Internships

During his three years of study at the Higher School of Health Sciences and Techniques of Sfax, he completed technician internships in various departments at the Habib BOURGUIBA University Hospital, covering hematology, biochemistry, bacteriology and virology, immunology, and parasitology. From 07/06 to 07/07/2000, he undertook an internship at the pharmaceutical company SIMED in Sfax, focusing on techniques of physico-chemical analysis and microbiological control. From 01/07 to 31/07/2001, he interned at the company AGROMED (Mamie Nova) in El Amra, working on the application of the HACCP system on the production chain of skimmed butter until the finished packaged product.

Additionally, he participated in various educational events and workshops, including the spring pedagogy university event at ENIS in April 2004, the Pedagogical Training Cycle at ENIS in April 2005, and the “First Tunisian-German Workshop on Bioinformatics” in Borj Cédria from 8-10 September 2006, where he presented a poster. He also attended the Training School on Experimental Designs at the University of Sfax from 22-24 March 2007 and participated in the pedagogical training seminar titled “The Place of Self-learning and Information and Communication Technologies” in May 2007, which was organized in collaboration with the Catholic University of Louvain (Belgium) as part of the European TEMPUS program, held at the Higher Institute of Biotechnology of Sfax.

 

📝🔬Publications📝🔬

 

Ahlam H. Mahmoud – Biochemistry – Best Researcher Award

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Ahlam H. Mahmoud – Biochemistry

Prof Dr. Ahlam H. Mahmoud distinguished academic and researcher in the field Biochemistry. Ahlam Hosny Mahmoud has a rich and extensive employment history in the field of biochemistry and therapeutic chemistry. Beginning in 1987, he served as a research assistant in the Therapeutic Chemistry Department within the Pharmaceutical and Drug Industries Research Division at the National Research Centre in Dokki, Giza, Egypt.

Eduvation

Ahlam has dedicated his career to advancing research in the pharmaceutical and drug industries, contributing significantly to the scientific community. Currently, he holds the esteemed position of Professor of Biochemistry within the same department at the National Research Centre in Dokki, Giza, Egypt. As a professor, he continues to make substantial contributions to the field, shaping the future of biochemistry and therapeutic chemistry through his research and academic endeavors.
Professional Profiles:

EXPERIENCE

In his pursuit of advancing pharmaceutical research, Ahlam Hosny Mahmoud has engaged in a variety of in vivo experiments involving experimental animals. These experiments are designed with the overarching goal of developing new drugs or identifying new drug indications. One facet of his research involves conducting in vivo experiments on gastric ulcer and inflammatory diseases of soft tissue. In these studies, he meticulously induces diseases in experimental animals and evaluates the efficacy of treatments, with a specific focus on assessing the degree of inflammation using a planimeter.

Furthermore, Ahlam is actively involved in in vivo treatments targeting breast cancer. His efforts contribute to the ongoing exploration of novel approaches for combating this prevalent and challenging disease. Additionally, he undertakes experiments aimed at measuring the toxicity of different compounds, providing crucial insights into the safety profile of potential therapeutic agents. Beyond that, Ahlam investigates the distribution and concentration of trace elements in various organs, shedding light on their potential roles in physiological processes and offering valuable information for drug development. Through these diverse in vivo experiments, he remains dedicated to advancing the frontiers of pharmaceutical and therapeutic research.

Project collaboration:

Principal investigator: The potential therapeutic effect of aqueous methanol extract of Bombex ceiba flower against breast cancer in female rats.

**Member: Using modern generations of formulations to prepare safe pesticides from natural materials for pest management in clean agriculture

Publication
  • Development of a New Benzofuran–Pyrazole–Pyridine-Based Molecule for the Management of Osteoarthritis
    Abd El-Karim, S.S., Mahmoud, A.H., Al-Mokaddem, A.K., …Zen, A.A., Anwar, M.M.
    MoleculesThis link is disabled., 2023, 28(19), 6814
  • Calcium voltage-gated channel subunit alpha 1 C and glial fibrillary acidic protein signaling pathways as a selective biomarker in predicting the efficacy of liposomal loaded co-enzyme Q in the autistic rat model
    Elhefnawei, D.M., Mahmoud, A.H., Kadry, M.O., …Badawy, M.A., EL-Desouky, M.A.
    Toxicology ReportsThis link is disabled., 2023, 10, pp. 17–26
  • Antiproliferative activity of bombax ceiba flower extract against mammary gland carcinoma in rats
    Mahmoud, A.H., Metwally, N.S., Youness, E.R., …Elmalt, H.A., Al-Mokaddem, A.K.
    Systematic Reviews in Pharmacy, 2020, 11(11), pp. 1406–1415
  • Synthesis and evaluation of new coumarin derivatives as antioxidant, antimicrobial, and anti-inflammatory agents
    Alshibl, H.M., Al-Abdullah, E.S., Haiba, M.E., …Bari, A., Villinger, A.
    MoleculesThis link is disabled., 2020, 25(14), 3251
  • Phytoconstituents and hepatoprotective effect of Suaeda monoica Forssk and Suaeda pruinosa Lange
    Elsharabasy, F.S., Metwally, N.S., Mahmoud, A.H., …Farrag, A.H., Arafa, S.
    Biomedical and Pharmacology JournalThis link is disabled., 2019, 12(1), pp. 117–129
  • Secondary metabolites and biological activities of allium porrum l. In attacking ehrlich ascites carcinoma in mice
    Mahmoud, A.H., Foda, D.S., Ibrahim, N.E., El-Hagrassi, A.M., Yousef, O.M.H.
    Egyptian Journal of ChemistryThis link is disabled., 2019, 62, pp. 211–227
  • A comparative study of the anticancer activity and PARP-1 inhibiting effect of benzofuran-pyrazole scaffold and its nano-sized particles in human breast cancer cells
    Anwar, M.M., El-Karim, S.S.A., Mahmoud, A.H., Amr, A.E.-G.E., Al-Omar, M.A.
    MoleculesThis link is disabled., 2019, 24(13), 2413
  • Bombax ceiba flowers extract ameliorates hepatosteatosis induced by ethanol and relatively moderate fat diet in rats
    Arafa, A.F., Foda, D.S., Mahmoud, A.H., Metwally, N.S., Farrag, A.R.H.
    Toxicology ReportsThis link is disabled., 2019, 6, pp. 401–408